Thermo Fisher, AM1753, Amino Allyl MessageAmp™ II aRNA Amplification Kit

Quantity: 20 Reactions
Unit Size: Each
Improved first- and second-strand cDNA synthesisIncluded in the kit is ArrayScript™ RT, a rationally engineered M-MLV reverse transcriptase, which produces equivalent or higher yields of full-length cDNA compared to other enzymes. The second-strand cDNA synthesis reaction has also been optimized to be compatible with the first-strand cDNA products generated with ArrayScript RT to allow maximal conversion of first-strand cDNA into full-length double-stranded cDNA templates.Increased labeling efficiency of your aRNAThe Amino Allyl MessageAmp II aRNA Amplification Kit incorporates amino allyl NTP into the aRNA followed by the coupling of its reactive amino group to an NHS ester label (e.g., Cy or another dye). This strategy offers several advantages over the direct incorporation of labeled NTPs. Direct incorporation of labeled NTPs is inefficient and results in low yields and low specific activity aRNA and high costs. Unlike lableled NTPs, amino allyl–modified NTPs are incorporated almost as efficiently as unmodified NTPs and are much less expensive than the dye coupled NTPs.
• Kit includes sufficient reagents for 20 reactions
• Cy™ dyes are not includedExpression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, RNA Amplification