Thermo Fisher, AM1908, MEGAclear™ Transcription Clean-Up Kit

Quantity: 20 Preps
Unit Size: Each
The MEGAclear kit purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components, ideal for any downstream application that requires purified RNA. It can be used to remove nucleotides, short oligonucleotides,proteins, and salts from RNA. The RNA recovered can be used for any application that requires high purity RNA.Use the MEGAclear lit to clean up any of the following:
• In vitro transcribed RNA:RNA from MEGAscript reactionsAmino allyl-modified RNABiotinyled RNACy dye labeled RNACapped RNA (e.g., from mMESSAGE mMACHINE kit reactions)
• Total RNAUsing the MEGAclear kitThe protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitro transcribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.