Thermo Fisher, AM2542, Proteinase K Powder, 100 mg

Name: Proteinase K Powder, 100 mg
Brand: Thermo Fisher
SKU: AM2542

Quantity: 100 mg, Unit Size: Each

Catalog Number: AM2542

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Category

Recombinant Enzymes

Pack Size100 mg

TagsModifying Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 100 mg
Unit Size: Each
Proteinase K from the fungus Engyodontium album is a non-specific serine protease that is useful for general digestion of proteins.Proteinase K Remains Active:• Over a wide pH range (optimal activity between 6.5 and 9.5)• Under denaturing conditions (e.g. the presence of SDS or urea)• In the presence of metal chelating agents (e.g. EDTA)• At comparatively high temperatures (optimum digestion temperature is 65°C)Applications: Removal of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for in situ hybridization.Performance and Quality Testing: Endodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.Unit Definition: One mAnson unit is described as that amount of enzyme that liberates 1 μmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate.

Specifications

General

Host Species	T. album
Packaging	Bottle
Product Type	Proteinase K
Quantity	100 mg
Shipping Condition	Dry Ice
Product Line	Ambion
Unit Size	Each

FAQs

Question: What is the supplied amount?

Answer:The product contains 100 mg of lyophilized Proteinase K powder.

Question: Can Proteinase K remain active in SDS or urea?

Answer:Yes. The enzyme remains active in the presence of SDS or urea.

Question: How should the dry enzyme be stored?

Answer:It should be stored at –20°C with desiccation and warmed to room temperature before opening.

Application Note

Proteinase K Powder, 100 mg is used to digest proteins during nucleic-acid sample preparation and to inactivate protein enzymes after biochemical treatments. This nonspecific serine protease remains active in the presence of SDS or urea and functions across broad ranges of pH, salt concentration, and temperature, making it useful for demanding lysis and cleanup workflows. The enzyme can support preparation of RNA and high-molecular-weight DNA, including DNA intended for pulsed-field gel electrophoresis. It can also terminate phosphatase or nuclease treatments by degrading the enzyme responsible for the preceding reaction. In samples containing high protein concentrations, Proteinase K digestion can improve subsequent phenol extraction by reducing protein burden. This product is supplied as a lyophilized powder isolated from Tritirachium album and is stored at –20°C with desiccation. Warming the sealed container to room temperature before opening helps protect the dry enzyme from moisture condensation.

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