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This 100X semi-apochromat (FL) Olympus objective has exceptional optical performance and is suitable for a wide range of applications. These objectives deliver superb brightness and resolution across a large field of view for applications where high image quality is desired. Olympus objectives are compatible with all EVOS cell imaging systems. Our wide selection of EVOS and Olympus objectives satisfies needs across the spectrum of magnifications and optical specifications. Additional characteristics of this Olympus objective: • Magnification: 100X • Numerical Aperture: 0.95 • Working Distance: 0.2 mm • Immersion: oil Image quality Microscope objectives may be the most important components of a microscope because they are responsible for primary image formation. High image quality is crucial to experimental success and often a requirement for publication; Olympus objectives afford that quality across the visible spectrum to near infrared light. This performance results from years of lens manufacture experience, and the broad selection of objectives means that you can choose the optimal objective for your imaging requirements. Objective classes Achromat objectives are perfect for general applications, with standard correction of color and focus. Fluorite objectives deliver excellent resolution and are made with higher numerical apertures than achromat objectives, resulting in brighter fluorescence signal and higher contrast imaging. The higher optical quality greatly reduces optical aberrations, and corrections for color and focus are at higher levels than achromat objectives. Fluorite objectives are ideally suited for fluorescence and demanding transmitted light applications, where the higher contrast make them ideally suited for color imaging. Apochromat objectives are manufactured to the highest levels of resolution, fluorescence brightness, and contrast; chromatic aberrations are almost eliminated. They are recommended for the most demanding applications, particularly at magnifications of 60x and above. Apochromatic objectives are the best choice for the capture of color images in white light. Brightfield contrast versus phase contrast objectives Brightfield is the most basic form of light microscopy and is accomplished by sample absorption of light. A higher density area in a sample will absorb more light, thus increasing contrast in those areas. Phase contrast objectives are most useful for hard to see, translucent specimens. This method of contrast is accomplished by converting phase shifts, caused by light passing through a translucent specimen, into brightness changes (i.e., contrast). Long working distance versus coverslip-corrected objectives Long working distance (LWD) objectives are optimized for use through vessels with a nominal wall thickness of 0.9–1.5 mm. This includes vessels commonly used in cell culture and cell-based assays, such as slides, cell culture dishes and flasks, microtiter well plates, etc. Coverslip-corrected objectives are optimized for use through #1.5 coverslips (thickness approximately 0.17 mm). These objectives have a higher magnification/NA ratio and provide higher resolution compared to LWD objectives. For additional choices, visit the EVOS and Olympus objectives selection guide.
