Thermo Fisher, BP172-5, Dithiothreitol (White Crystals or Powder/Electrophoresis), Fisher BioReagents™

Name: Dithiothreitol (White Crystals or Powder/Electrophoresis), Fisher BioReagents™
Brand: Thermo Fisher
SKU: BP172-5

Quantity: 5 g, Packaging: Glass Bottle, Unit Size: Each, Dithiothreitol, >-99%, C4H10O2S2, CAS Number-3483-12-3, 1, 4-dithio-dl-threitol, 1, 4-dithiothreitol, 2s, 3s-1, 4-dimercaptobutane-2, 3-diol, d-1, 4-dithiothreitol, d-dtt, dithiothreitol, dl-1, 4-dithiothreitol, dl-dithiothreitol, dtt, threo-1, 4-dimercapto-2, 3-butanediol, 5g, 0.04 AU max., >-99 %

Catalog Number: BP172-5

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General

Absorbance	0.04 AU max.
Assay Percent Range	≥99 %
CAS	12-3-3483
ChEBI	CHEBI:42170
ChemAlert Storage Symbol	Gray
Chemical Name or Material	Dithiothreitol
Color	White
Formula Weight	154.24
IUPAC Name	(2S,3S)-1,4-bis(sulfanyl)butane-2,3-diol
InChI Key	VHJLVAABSRFDPM-IMJSIDKUSA-N
Linear Formula	HSCH<sub>2</sub>CH(OH)CH(OH)CH<sub>2</sub>SH
Melting Point	38°C
Merck Index	15, 3419
Molecular Formula	C4H10O2S2
Packaging	Glass Bottle
PubChem CID	446094
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	-20°C
SMILES	C(C(C(CS)O)O)S
Synonym	threo-1,4-dimercapto-2,3-butanediol
Boiling Point	125°C
Grade	Electrophoresis
Molecular Weight (g/mol)	154.24
Percent Purity	≥99%
Physical Form	Powder or Solid
Quantity	5 g

FAQs

Question: What is Fisher BioReagents dithiothreitol used for?

Answer:DTT reduces disulfide bonds and helps maintain proteins and enzymes in a reduced state during biochemical workflows.

Question: Is the Fisher BioReagents DTT product a liquid solution?

Answer:No. This 5 g product is supplied as white crystals or powder.

Question: How should the DTT reagent be stored?

Answer:The product specification recommends storage at −20 °C.

Application Note

Fisher BioReagents™ dithiothreitol (DTT) is a thiol-based reducing agent supplied as 5 g of white crystals or powder in a glass bottle. The reagent has an assay of at least 99% and is specified as free from detectable DNase, RNase and protease activity. DTT reduces disulfide bonds and helps maintain sulfhydryl groups in their reduced state during protein extraction, electrophoresis, enzyme preparation and selected molecular biology workflows. Its reducing action is time- and condition-dependent, so prepare concentrations that match the protocol and avoid unnecessary exposure of working solutions to air. This material is a solid reagent rather than a premixed reduction buffer. The manufacturer's recommended storage temperature is −20 °C. Control moisture exposure and use the current safety data sheet when weighing and dissolving the chemical.

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