Thermo Fisher, E33856, EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit

Quantity: 1 Kit
Unit Size: Each
Myeloperoxidase (MPO) is unique peroxidase that, in addition to its peroxidation activity, also catalyzes conversion of hydrogen peroxide (H_2O_2) and chloride (Cl^-) to hypochlorous acid (HOCl). The EnzChek™ Myeloperoxidase (MPO) Activity Assay Kit provides assays for determination of both chlorination and peroxidation activities of MPO in solution and in cell lysates.
• For detection of chlorination, kit includes nonfluorescent 3'-(p-aminophenyl) fluorescein (APF), which is selectively cleaved by hypochlorite (^-OCl) to yield fluorescein Peroxidation is detected using nonfluorescent AmplexUltraRed reagent (A36006), which is oxidized by H_2O_2-generated redox intermediates MPO-I and MPO-II to form fluorescent product
• EnzChek Myeloperoxidase Activity Assay Kit can be used to continuously detect these activities at room temperature over broad dynamic range (1.5 to 200ng/mL)
• Kit combines speed (30 minutes), sensitivity and mix-and-read convenienceCell Analysis, Cell Viability, Proliferation and Function, Cellular Imaging, Enzyme and Protein Activity Assays, High-Content Screening (HCS), Nitro-Oxidative Stress, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis, Reactive Oxygen Species