Thermo Fisher, EN0542, RNase T1 (1000 U/μL), 500,000 units

Name: RNase T1 (1000 U/μL), 500,000 units
Brand: Thermo Fisher
SKU: EN0542

Quantity: 500, 000 units, Unit Size: Each, Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.Rnase T1 does not require metal ions for activi

Catalog Number: EN0542

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General

Product Type	RNase T1
Quantity	500,000 units
Concentration	1000 U/μL
Enzyme	RNase
Unit Size	Each

FAQs

Question: Where does RNase T1 cleave RNA?

Answer:RNase T1 cleaves single-stranded RNA at guanosine residues, generating fragments with terminal guanosine phosphates.

Question: What concentration and total activity are supplied?

Answer:The enzyme is supplied at 1,000 U/µL with 500,000 units of total activity.

Question: Does RNase T1 require a metal cofactor?

Answer:No. Its ribonuclease activity does not require metal ions.

Application Note

Thermo Scientific RNase T1 is a guanosine-specific endoribonuclease used to cleave single-stranded RNA after guanosine residues. The enzyme produces RNA fragments with terminal guanosine phosphates and does not require metal ions for catalytic activity. This formulation contains 1,000 U/µL, with 500,000 units supplied in total. RNase T1 supports controlled RNA digestion, removal of RNA from DNA or protein preparations, and ribonuclease protection experiments, often alongside RNase A. Because it selectively cleaves accessible RNA sequences, reaction design should account for substrate structure, enzyme concentration and digestion conditions.

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