Thermo Fisher, FD1134, FastDigest SacI, 200 μL (200 reactions)

Name: FastDigest SacI, 200 μL (200 reactions)
Brand: Thermo Fisher
SKU: FD1134

Quantity: 200 μL (200 reactions), Unit Size: Each, FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Ligase, alkaline phosphatases and T4 DNA Polymerase all have 100%

Catalog Number: FD1134

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Category

Restriction Enzymes

Pack Size200 µL

TagsRestriction Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 200 μL (200 reactions)
Unit Size: Each
Thermo Scientific FastDigest SacI restriction enzyme recognizes GAGCT^C site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer. Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI.Thermo Scientific FastDigest SacI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.Features• 100% activity of all FastDigest enzymes in the universal buffer• 100% buffer compatibility with downstream applications• Complete digestion in 5–15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism (RFLP)• SNP analysisNote: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.For methylation sensitivity, refer to product specifications.

Specifications

General

Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Inactivation Temperature	65°C
Overhang	3' protruding ends
Product Type	Restriction Enzyme
Quantity	200 μL (200 reactions)
Enzyme	SacI
Methylation Sensitivity	Not Dam Methylation-Sensitive
Optimal Reaction Temperature	37°C
Product Line	FastDigest
Research Category	Traditional Cloning
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Unit Size	Each

FAQs

Question: What DNA sequence does FastDigest SacI recognize?

Answer:It recognizes GAGCT^C and generates 3′ protruding ends.

Question: How many reactions are included?

Answer:This configuration provides approximately 200 reactions.

Question: Can the enzyme be heat inactivated?

Answer:Yes. FastDigest SacI is heat-inactivatable at 65°C.

Application Note

FastDigest SacI is used for rapid restriction digestion of DNA in cloning, mapping, genotyping and related molecular-biology applications. The enzyme recognizes the sequence GAGCT^C and generates 3′ protruding ends. It is an isoschizomer of Eco53kI, EcoICRI, Psp124BI and SstI. SacI reaches full activity at 37°C in the universal FastDigest and FastDigest Green buffers and can typically complete digestion in 5–15 minutes. This configuration contains 200 µL of enzyme for approximately 200 reactions and includes FastDigest reaction buffer. The enzyme can be heat inactivated at 65°C when a downstream workflow benefits from removing restriction activity without a separate purification step. The shared buffer system also supports efficient double or multiple digestion with compatible FastDigest enzymes.

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