Thermo Fisher, FD1414, FastDigest ApaI

Name: FastDigest ApaI
Brand: Thermo Fisher
SKU: FD1414

Quantity: 300 μL (300 Reactions), Unit Size: Each, Lambda DNA digested with ApaI, 0.7% agarose, 1 cleavage sitesFastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers.The universal buffer allows rapid single-, double- or multiple DNA digestion within 5-15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. DNA modifying enzymes, such as Klenow Fragment, T4 DNA Liga

Catalog Number: FD1414

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Category

Restriction Enzymes

Pack Size300 µL

TagsRestriction Enzymes Enzymes and Inhibitors

CollectionsThermo Fisher Enzymes & Inhibitors for PCR, Cloning & Protein Protection Thermo Fisher Clinical Analyzers & Instruments for Diagnostic Labs Thermo Fisher General Lab Supplies for Daily Workflows — Nalgene, Nunc, Samco & Matrix

Description

Quantity: 300 μL (300 Reactions)
Unit Size: Each
The universal buffer allows rapid single-, double-, or multiple DNA digestion within 5–15 minutes eliminating any need for buffer change or subsequent DNA clean-up steps. See Reaction Conditions for FastDigest Enzymes for a table of enzyme activity, heat inactivation and incubation times for this and other FastDigest restriction enzymes. DNA modifying enzymes, such as Klenow Fragment (Cat. No. EP0051), T4 DNA Ligase (Cat. No. EL0014), alkaline phosphatases and T4 DNA Polymerase (Cat. No. EP0061) all have 100% activity in FastDigest Buffer. Therefore, enzymes for downstream applications can be directly added to the FastDigest reaction mix.For additional convenience FastDigest Green Buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal FastDigest Buffer eliminate star activity effects.Features• 100% activity of all FastDigest enzymes in the universal buffer• 100% buffer compatibility with downstream applications• Complete digestion in 5–15 minutes• Direct loading on gels• No star activity• 176 FastDigest enzymes availableApplications• Molecular cloning• Restriction site mapping• Genotyping• Southern Blot• Restriction fragment length polymorphism (RFLP)• SNP analysisNote: The FastDigest Green Buffer offers the same high performance in DNA digestion and downstream applications as the colorless FastDigest Buffer. For applications that require product analysis by fluorescence excitation (e.g. concentration measurements in UV light) the colorless FastDigest Buffer is recommended.For methylation sensitivity, refer to product specifications.

Specifications

General

Compatible Buffer	10X FastDigest Buffer/FastDigest Green Buffer
Inactivation Temperature	65°C
Overhang	3' protruding ends
Product Type	Restriction Enzyme
Quantity	300 μL (300 Reactions)
Enzyme	Apa I
Methylation Sensitivity	CpG Methylation-Sensitive
Optimal Reaction Temperature	37°C
Product Line	FastDigest
Research Category	Traditional Cloning
Sensitive to Heat Inactivation	Yes
Type IIS RE	No
Unit Size	Each

FAQs

Question: What sequence is recognized by FastDigest ApaI?

Answer:It recognizes GGGCC^C and generates 3′ protruding ends.

Question: What reaction temperature is recommended?

Answer:The enzyme is used at 37°C.

Question: How many standard reactions are included?

Answer:This configuration provides approximately 300 reactions.

Application Note

FastDigest ApaI is used for rapid restriction digestion in cloning, restriction mapping, genotyping and other DNA-manipulation workflows. The enzyme recognizes the sequence GGGCC^C and produces 3′ protruding ends. ApaI performs optimally at 37°C and can typically complete digestion in 5–15 minutes in universal FastDigest or FastDigest Green Buffer. This configuration contains 300 µL of enzyme for approximately 300 reactions, making it suitable for laboratories with repeated digestion requirements. The common FastDigest buffer system simplifies double and multiple digestions because compatible enzymes can be combined without sequential buffer exchange. FastDigest Green Buffer can be loaded directly onto agarose gels, while the colorless buffer remains useful when downstream fluorescence-based measurements or other dye-sensitive analyses are planned.

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