| Color | Green |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Hot Start | No |
| No. of Reactions | 1000 Reactions |
| Overhang | 3'-A |
| Polymerase | DreamTaq DNA Polymerase |
| Quantity | 20 x 1.25 mL |
| Reaction Format | SuperMix or Master Mix |
| Shipping Condition | Dry Ice |
| Concentration | 2X |
| For Use With (Application) | Standard PCR |
| GC-Rich PCR Performance | Low |
| Reaction Speed | Standard |
| Unit Size | Each |
Answer:The 2X mix contains DreamTaq DNA polymerase, reaction buffer, MgCl2, dNTPs, density reagent and tracking dyes.
Answer:Yes. The Green formulation contains loading-density reagent and tracking dyes, allowing direct gel loading after amplification.
Answer:Under suitable PCR conditions, the mix can amplify genomic DNA targets up to 6 kb and viral DNA targets up to 20 kb.
Answer:No. DreamTaq Green PCR Master Mix is a conventional PCR master mix rather than a hot-start formulation.
Answer:This package supports approximately 1000 reactions and contains 20 tubes of 1.25 mL each.
DreamTaq™ Green PCR Master Mix (2X) is a ready-to-use mixture for routine endpoint PCR. It contains DreamTaq DNA polymerase, optimized buffer, MgCl2 and dNTPs; the Green formulation also contains density reagent and tracking dyes so amplified samples can be loaded directly onto an agarose gel after PCR. The mix can amplify genomic DNA targets up to 6 kb and viral DNA targets up to 20 kb under suitable PCR conditions. The master mix is suited to common genomic and plasmid amplification, genotyping, colony PCR, clone screening and other standard PCR workflows where streamlined reaction setup is useful. This configuration contains 20 × 1.25 mL and supports approximately 1000 standard reactions. Store at −20°C and use for research applications.
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