Thermo Fisher, K156001, PureLink™ Total RNA Blood Kit

Quantity: 50 preps
Unit Size: Each
PureLink Total RNA Blood Kit allows purification of high-quality total RNA from 50 to 500 μL fresh, whole mammalian blood, using non-phenol based purification in a simple spin column based protocol.Simple RNA Purification from Whole Blood PureLink Total RNA Blood Kit is designed for purifying from 50 to 500 μL fresh whole blood or approximately 3.5 x 10^6 leukocytes (white blood cells) in less than 1 hour. The entire protocol can be done in spin tubes and spin columns.Leukocyte Enrichment for Improved RNA Purification Whole blood consists of non-nucleated erythrocytes (red blood cells, 4 - 6 million/mm^3) and leukocytes, with the latter present in the minority (5000 - 7000/mm^3). Purifying total RNA from whole blood without the removal of erythrocytes results in low RNA yields and clogging of purification columns. The distinct feature of the PureLink Total RNA Blood Kit is that it employs a leukocyte enrichment step. Hypotonic shock is used to selectively lyse erythrocytes while not affecting leukocytes. Non-nucleated erythrocytes are efficiently depleted, thus significantly reducing cell debris that may otherwise interfere with RNA purification and other downstream applications. Consequently, yields of high-quality total RNA are obtained using the PureLink Total RNA Blood Kit.
• RNA purification from whole blood in under an hour
• Yields high-quality RNA devoid of non-nucleated erythrocytesPurified RNA is Suitable for Use with a Variety of Downstream Applications and Products:
• Northern Blotting Kits & Reagents
• Nuclease protection assays
• Microarray
• cDNA synthesis
• Real-Time PCR (qPCR)Not high-throughput compatible (Manual)ApplicationsDNA and RNA Purification and Analysis, RNA Extraction, Total RNA Isolation, Total RNA from Blood