Thermo Fisher, K244020, pcDNA™3.2/GW/D-TOPO™ Expression Kit

Quantity: 20 reactions
Unit Size: Each
• Cytomegalovirus (CMV) promoter for high-level expression
• Adapted for Directional TOPO Cloning, enabling to use proofreading polymerases and to clone PCR products in specific orientation
• Neomycin resistance gene for stable selection
• C-terminal V5 tag for easy detection
• Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
• TOPO Cloning: Using restriction enzymes to clone gene into an expression vector often forces to compromise final sequence of insert, especially when there are no useful restriction sites close to genes coding sequence, which may result in suboptimal spacing of expression elements or incorporation of non-native amino acid residues, reducing expression levels and/or cause production of non-functional protein
• In addition to being more effective way to clone, TOPO Cloning eliminates these potential expression problems
• TOPO Expression Vectors enable to insert exact DNA sequence require simply by performing PCR with appropriately designed primers
• The PCR product is cloned at high efficiency in five minutes into topoisomerase I-activated expression vector
• Resulting recombinant expression vector contains exact DNA sequence without any noncoding regionsCloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis