Thermo Fisher, K30001, Champion™ pET SUMO Expression System

Quantity: 20 reactions
Unit Size: Each
• Uses small ubiquitin-related modifier (SUMO) fusion, belonging to growing family of ubiquitin-related proteins, to enhance solubility of expressed fusion proteins
• Contrast to ubiquitin, SUMO is involved in stabilization and localization of proteinsin vivo
• After expression, 11 kd SUMO moiety can be cleaved by highly specific and active SUMO (ULP-1) protease at carboxyl terminal, producing native protein
• Enhanced solubility with N-terminal SUMO fusion
• Highly efficient cleavage produces native protein of interest with SUMO (ULP-1) protease
• Highly specific cleavage eliminates chance of protein of interest being internally digested, regardless of its amino acid sequence
• Significantly increased stability with SUMO fusion-can be used for small peptide productionT7lac promoter for high-level protein expression
• N-terminal 6xHis tag for protein detection and purificationBacterial Expression, Protein Expression, Proteins, Expression, Isolation and Analysis