| Chemical Name or Material | Running Buffer |
|---|---|
| Formulation | 1X: 50 mM MES, 50 mM Tris Base, 0.1% SDS, 1 mM EDTA, pH 7.3 |
| Recommended Storage | <b>Contents:</b> NuPAGE™ MES SDS Running Buffer (20X)<br /><b>Storage:</b> +4°C to 25°C |
| Solubility Information | Water |
| Concentration | 20X |
| Physical Form | Liquid |
| Product Line | NuPAGE |
| Quantity | 500 mL |
| pH | 7.3 |
| Unit Size | Each |
Answer:The 1X buffer contains 50 mM MES, 50 mM Tris base, 0.1% SDS and 1 mM EDTA at approximately pH 7.3.
Answer:It is designed for NuPAGE Bis-Tris gels and can also be used with compatible Bolt Bis-Tris gel workflows.
Answer:Store the concentrate at 4–25 °C.
NuPAGE™ MES SDS Running Buffer (20X), 500 mL is used for SDS-PAGE of small-to-medium proteins with NuPAGE Bis-Tris gels. Dilution to 1X produces a running buffer containing 50 mM MES, 50 mM Tris base, 0.1% SDS and 1 mM EDTA at approximately pH 7.3. The MES-based system provides the ionic environment for electrophoretic separation in the NuPAGE Bis-Tris workflow and is supplied as a 500 mL, 20X liquid concentrate for preparation of working buffer as needed. The buffer can also be used with compatible Bolt Bis-Tris gel workflows. Store the concentrate at 4–25 °C. This format is suited to routine protein electrophoresis where a defined MES/SDS buffer system is required for separation of small-to-medium proteins.
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