Thermo Fisher, R0861, DTT (dithiothreitol), 5 g

DTT (dithiothreitol), 5 g is used for reducing disulfide bonds and maintaining sulfhydryl groups in proteins and enzymes during biochemical experiments. This DL-dithiothreitol powder acts as a thiol-based reducing reagent and can restore activity lost when sensitive free sulfhydryl groups become oxidized in vitro. It quantitatively reduces disulfide bonds and helps maintain monothiols in their reduced state under suitable conditions. The product is recommended for enzyme stabilization, enzymatic reactions and protein electrophoresis. A final concentration of approximately 0.1 M is used for disruption of protein disulfide bonds in certain SDS–polyacrylamide gel electrophoresis workflows, although the concentration must match the protocol. This exact package contains 5 g of DTT powder. The reagent is tested to be free of detectable endonucleases, exonucleases, ribonucleases and phosphatases. Prepare fresh solutions when needed because dissolved DTT has limited stability. The solid reagent is suitable for laboratories needing a defined reducing agent for proteins and other protocols that specify dithiothreitol rather than an unrelated reductant.