Thermo Fisher, S1262, SBFI, Tetraammonium Salt, cell impermeant

Quantity: 1 mg
Unit Size: Each
• Useful in combination with other fluorescent indicators used to correlate changes in intracellular Na^+with Ca^2+and Mg^2+concentrations, intracellular pH, and membrane potential
• Although selectivity of SBFI for Na^+is less than that of calcium indicators such as fura-2, it is sufficient for detection of physiological concentrations of Na^+in presence of other monovalent cations
• The spectral response of SBFI upon ion binding permit excitation ratio measurements, and this indicator can be used with same optical filters and equipment used for fura-2
• Label (Ex/Em): SBFI (approximately 340,380/500nm)
• Lyophilized product may be dissolved in distilled water or aqueous buffer for use
• Product is typically loaded into cells by diffusion from patch pipet for correlated fluorescence imaging and electrophysiological recording
• Dissociation constant (K_d) of SBFI for Na^+is 3.8mm in the absence of K^+, and 11.3 mm in solutions with combined Na^+and K^+concentration of 135 mm (which approximates physiological ionic strength). SBFI is approximately 18-fold more selective for Na^+than for K^+Cell Analysis, Cell Metabolism, Cell Viability, Proliferation and Function, Ionic Homeostasis and Signaling, Sodium and Potassium Detection