Tosoh, 0008068, TOYOPEARL HW-65S

TOYOPEARL HW-65S resin is a hydroxylated methacrylic polymer for size exclusion chromatography. TOYOPEARL HW-65S resin has a 100 nm pore size and a 30 µm bead size. Surface hydroxyl groups render this resin very hydrophilic, therefore minimal non-specific adsorption occurs, making the TOYOPEARL HW-65S resin useful for protein separations of biomolecules between 40,000 and 5,000,000 Da in size. The good mechanical stability of the TOYOPEARL HW-65S resin produces excellent flow characteristics in large industrial size columns (up to 0.3 MPa). TOYOPEARL HW-65S resin is chemically stable from pH 2-14, and it is this stability that allows a constant packing volume over a wide range of salt concentrations and cleaning in place (CIP) with acid or base. Also, this resin can be run at elevated temperatures (4 - 60 °C) and is autoclavable at 121 °C. If lower resolution is needed, the larger particle TOYOPEARL HW-65F resin can also be used for process SEC.