Tosoh, 0013066, TSKgel Boronate-5PW

TSKgel Boronate-5PW is based on a 100 nm pore size polymethacrylate base material bonded with m -aminophenyl boronate. This ligand is capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis -diol groups such as those found in carbohydrates, glycosylated proteins, and catecholamines. Interaction between the boronate anion and the 1,2 cis -diol groups is enhanced in the presence of Mg 2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers, such as HEPES and morpholine, while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris. Applications include nucleic acids, catecholamines, nucleosides, and nucleotides.