Tosoh, 0013071, TSKgel Amide-80, 5 µm

Name: TSKgel Amide-80, 5 µm
Brand: Tosoh
SKU: 0013071

TSKgel Amide-80, 5 µm columns are packed with spherical silica particles that are covalently bonded with carbamoyl groups.

Catalog Number: 0013071

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Category

HILIC Columns

TagsAnalytical Columns Hydrophilic Interaction - HILIC Normal Phase

Description

TSKgel Amide-80, 5 µm columns are packed with spherical silica particles that are covalently bonded with carbamoyl groups. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography. TSKgel Amide-80, 5 µm columns possess superior stability in aqueous/organic solvent systems, an advantage over other hydrophilic stationary phases such as traditional amino-bonded phase columns. In addition, TSKgel Amide-80, 5 µm columns are ideally suited for sensitive LC/MS analysis of water-soluble polar compounds because the high organic content in the mobile phase provides rapid evaporation of solvent during electrospray ionization. Target applications for the TSKgel Amide-80, 5 µm columns include the analysis of saccharides, glycans, oligosaccharides, peptides, and polar compounds from natural products. The pore size of the bonded phase is indicated by the number in the product description, in this case TSKgel Amide-80 has 8 nm nominal pore size. The nominal pore size of the starting base silica is 10 nm.

Specifications

General

Column Diameter (mm)	2, 1, 4.6
Column Length (cm)	10, 25, 15, 5
Housing Material	Stainless Steel
Particle Size (µm)	5
Pore Size (nm)	10
Mode	Normal Phase/HILIC
Product Category	HPLC Columns
Group	Specialty
Base Material	Silica

FAQs

Question: What are the dimensions of this version?

Answer:This column is 4.6 mm ID × 250 mm long.

Question: What stationary phase is used?

Answer:It contains 5 µm carbamoyl-bonded silica with nominal 80 Å pores.

Question: What samples can be analyzed?

Answer:Typical uses include glycans, sugars, oligosaccharides, and other polar analytes in HILIC.

Application Note

TSKgel Amide-80 is a silica-based HILIC column for analysis of polar compounds that show limited retention on typical reversed-phase stationary phases. This specific analytical configuration measures 4.6 × 250 mm and uses 5 µm spherical silica particles bonded with carbamoyl groups. The bonded amide phase has a nominal pore size of approximately 80 Å and offers retention and selectivity for carbohydrates, glycans, oligosaccharides, polar metabolites, and related water-soluble analytes. Its chemical stability in mixed aqueous and organic mobile phases makes it useful for LC–MS methods with high organic-solvent content. The 250 mm column length provides resolving power for closely related hydrophilic species. This SKU is the 5 µm analytical column, not an Amide-80 HR variant, bulk gel, or guard cartridge.

Ordering

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