Tosoh, 0014710, TSKgel DEAE-5PW (20)

TSKgel DEAE-5PW (20) resin is a weak anion exchange resin for biomolecule purification. The resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with diethyl amino ethyl (DEAE) weak anion exchange groups. This resin has a 100 nm pore size and a 20 µm bead size. TSKgel DEAE-5PW (20) resin has a high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW ion exchange chromatography resins. A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material, however; chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. In this way, they are higher performing chromatography resins than their TOYOPEARL counterparts. TSKgel DEAE-5PW (20) resin is best used for high resolution process polishing steps. If a product with less resolution is needed, the larger particle TSKgel SP-5PW (30), TOYOPEARL DEAE-650, or TOYOPEARL GigaCap DEAE resins may be used. Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.