Tosoh, 0030004, TSKgel SuperQ-4PW (20)

TSKgel ® SuperQ-4PW (20) resin is a strong anion exchange resin for biomolecule purification. This resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with quaternary amine (Q) strong anion exchange groups. This resin has a 50 nm pore size and a 20 µm bead size. TSKgel SuperQ-4PW (20) resin is best used for high-resolution process polishing steps and the capture of short oligonucleotides (<50-mers) from crude harvest. This resin’s 50 nm pore size is optimized for higher binding capacity and improved selectivity of shorter oligos. If a longer oligonucleotide (>50-mers) is needed, the larger pore-sized TSKgel SuperQ-5PW (20) may be used. TSKgel SuperQ-4PW (20) resin has high chemical and mechanical stability, allowing cleaning-in-place (CIP) and high flow rates. TSKgel PW resins vary from TOYOPEARL resins by having a higher degree of crosslinking which is necessitated by the higher flow pressures needed when using smaller particles for chromatography. The increased level of crosslinking decreases the number of sites available for ligand immobilization when producing TSKgel PW ion exchange chromatography resins. A TSKgel PW resin will have a lower dynamic binding capacity than the corresponding TOYOPEARL material, however chromatographic efficiency, peak width, and resolution are improved because of their smaller particle sizes. If a product with less resolution is needed, the larger particle-sized TOYOPEARL SuperQ-650 resins may be used. Because similarly functionalized TSKgel PW and TOYOPEARL resin types have the same backbone polymer chemistry, the selectivity for proteins and impurities remains the same.