Waters, 186004801, ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 1/pk

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ACQUITY UPLC BEH Amide is a hydrophilic-interaction chromatography column for retaining and separating highly polar compounds that show little retention on conventional reversed-phase phases. This 2.1 × 100 mm configuration uses 1.7 µm BEH particles with a trifunctionally bonded amide surface and 130 Å pores. The chemistry is useful for carbohydrates, sugars, nucleotide phosphates, and other polar analytes across a broad range of polarity and ionization behavior. The column supports UPLC operation at pressures up to 18,000 psi and can be used over a broad pH range, making it suitable for fast HILIC methods that need high efficiency and robust particle stability. Method development typically uses high-organic mobile phases with controlled aqueous content, followed by adequate equilibration to maintain retention reproducibility for strongly hydrophilic analytes.