Waters, 176006048, GTxResolve 2000Å SEC Column, MaxPeak Premier 3 μm, 4.6x300 mm and DNA Vector Mixture Standard
Catalog Number: 176006048
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Waters GTxResolve 2000Å SEC Columns, MaxPeak Premier 3µm are manufactured with low adsorption MaxPeak HPS hardware and novel bridged ethylene HO-PEO particles to support high recovery analyses of 800 to 2500Å diameter GTx drugs, such as plasmids, LNPs, and larger viral vectors. This column technology provides long column lifetimes, low secondary interactions, and improved MALS sensitivity. Facilitate the development of platform multiattribute SEC assays for plasmid integrity and LNP formulations analyses as well as improved detection of ejected transgenes and aggregates related to viral vectors. The Waters DNA Vector Mixture contains 25 µg of pBR322 dsDNA vector, 25 µg of Φ174 Virion ssDNA and 2 µg uracil (void marker). This standard can be used as a proficiency and system suitability test standard for chromatography separations, such as a size exclusion separation using a widepore 2000Å particle column.
| Chemistry | SEC |
|---|---|
| Separation Mode | SEC |
| Particle Substrate | Silica |
| pH Range Min | 2.5 pH |
| pH Range Max | 8 pH |
| Temperature Limits | 60 C |
| Maximum Pressure | 4000 psi (280 Bar) |
| Endcapped | No |
| Bonding Technology | SEC |
| Silanol Activity | Low |
| Particle Shape | Spherical |
| Particle Size | 3 µm |
| Endfitting Type | Parker-style |
| Pore Size | 2000 Å |
| Format | Premier Column |
| Surface Area | 19 |
| System | UHPLC, UPLC, HPLC |
| Particle Technology | Silica |
| Technique | SEC |
| USP Classification | L33 |
| Inner Diameter | 7.8 mm |
| Length | 300 mm |
| Carbon Load | 1 % |
| UNSPSC | 41115709 |
| Application | Gene Therapeutics |
| Brand | GTxResolve |
| Product Type | Columns |
| Units per Package | 1 pk |
Answer:This package contains a 4.6 mm ID, 300 mm long column rather than a 7.8 mm ID, 150 mm column.
Answer:It uses 2000 Å nominal-pore SEC packing with 3 µm particles.
Answer:Yes. The package includes the pBR322 dsDNA, ΦX174 ssDNA and uracil reference mixture.
The Waters GTxResolve 2000 Å size-exclusion chromatography package contains a MaxPeak Premier 3 µm SEC analytical column measuring 4.6 × 300 mm and a DNA Vector Mixture Standard. Its wide-pore packing and low-adsorption hardware support method development for large DNA vectors, plasmids, lipid nanoparticle formulations and related gene-therapy materials where nonspecific surface interactions can compromise recovery. The longer, narrower 300 mm column geometry differs from the 7.8 × 150 mm version and can affect resolution, flow rate, backpressure and solvent consumption. The accompanying reference mixture contains pBR322 double-stranded DNA, ΦX174 virion single-stranded DNA and uracil as a void marker. Use a mobile phase that preserves the assemblies being studied and verify the SEC operating range with appropriate standards. Characterize column performance through retention reproducibility, recovery, resolution and system suitability rather than transferring conditions unchanged from another diameter. When interfacing with multi-angle light scattering, evaluate dead volume and band broadening across the complete instrument flow path.
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