Waters, WAT011520, Ultrahydrogel 120 Column, 120 Å, 6 µm, 7.8 mm x 300 mm, 100 - 5K, 1/pk

Waters Ultrahydrogel Columns contain a sophisticated aqueous-compatible gel column for analytical and preparative separations of water-soluble polymers. The gel is a cross-linked hydroxylated polymer and contains some residual carboxyl functionality. Ultrahydrogel DP contains some residual amine functionality. Compounds normally characterized include oligomers and biological substances such as polysaccharides, nucleic acids, proteins, and peptides.

Waters Ultrahydrogel 120 is an aqueous size-exclusion chromatography column for separating relatively low-molecular-weight water-soluble polymers and selected biological molecules. The packed analytical column measures 7.8 mm internal diameter by 300 mm long and uses 6 µm cross-linked hydroxylated polymer particles with 120 Å nominal pore size. Its marketed molecular-weight fractionation range is approximately 100–5,000 Da, although the useful range and resolution depend on analyte chemistry and the calibration standards used. The aqueous gel packing is not a reversed-phase C18 medium and should not be selected for hydrophobic retention-based HPLC separations. Typical workflows include aqueous gel permeation chromatography of low-molecular-weight polysaccharide fractions and hydrophilic polymer distributions. Maintain compatible mobile-phase conditions, avoid abrupt solvent changes, and protect the analytical column with an appropriately matched guard cartridge when needed. Establish molecular-weight calibration with standards suited to the analyte and detector, and verify sample solubility before injection.