Waters, WAT011535, Ultrahydrogel 1000 Column, 1000 Å, 12 µm, 7.8 mm x 300 mm, 10K - 1M, 1/pk

Waters Ultrahydrogel Columns contain a sophisticated aqueous-compatible gel column for analytical and preparative separations of water-soluble polymers. The gel is a cross-linked hydroxylated polymer and contains some residual carboxyl functionality. Ultrahydrogel DP contains some residual amine functionality. Compounds normally characterized include oligomers and biological substances such as polysaccharides, nucleic acids, proteins, and peptides.

Waters Ultrahydrogel 1000 is a gel-filtration and aqueous size-exclusion chromatography column for higher-molecular-weight water-soluble analytes. Its 7.8 mm × 300 mm analytical format contains cross-linked hydroxylated polymer packing with a nominal pore size of 1000 Å and 12 µm particle size. The specified fractionation range is approximately 10,000 to 1,000,000 Da for suitable polymer standards, making the packing distinct from the smaller-pore Ultrahydrogel 120 column. The aqueous-compatible stationary phase can be used to characterize distributions of selected water-soluble polymers, polysaccharides and biological macromolecules when mobile-phase compatibility is established. Size-exclusion separations are sensitive to sample solubility, pore access and calibration chemistry; molecular-weight values are not universal across unrelated molecular conformations. Select a compatible aqueous mobile phase, avoid precipitation and unexpected secondary ionic interactions, and use an appropriate upstream guard configuration where necessary. The column is intended for chromatographic analysis and is not a bulk process chromatography resin.