Waters, 186007265, XBridge Glycan BEH Amide Column, 130Å, 2.5 µm, 2.1 mm X 150 mm, 1K - 150K, 1/pk

Name: XBridge Glycan BEH Amide Column, 130Å, 2.5 µm, 2.1 mm X 150 mm, 1K - 150K, 1/pk
Brand: Waters
SKU: 186007265

Catalog Number: 186007265

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Category

HILIC Columns

Pack Size1/pk

CollectionsWaters Service Parts & Kits for ACQUITY, Alliance & Arc Waters Sample Preparation: SPE, Syringe Filters & QuEChERS Waters Standards & Reagents for LC/MS, HPLC & GC

Description

Waters BEH-based Glycan chemistry offerings are available in three highly scalable particle sizes that address UPLC (i.e, 1.7 µm) and HPLC-based (2.5 µm XP and 3.5 µm) application needs. Each batch of BEH Glycan material is specifically quality control tested with the 2-AB labeled, Waters Glycan Performance standard to help ensure batch to batch consistency as well as highly similar separated glycan profiles. To help ensure highly similar results, chromatographers can now choose the most appropriate LC-based technology to address their specific released glycan analysis application needs and laboratory instrumentation.

Specifications

General

Chemistry	Amide
Separation Mode	Hydrophilic Interaction (HILIC)
Particle Substrate	Hybrid
pH Range Min	2 pH
pH Range Max	11 pH
Maximum Pressure	18000 psi (1240 Bar)
Endcapped	No
Silanol Activity	Low
Molecular Weight Range Min	1000
Molecular Weight Range Max	150000
Particle Shape	Spherical
Particle Size	2.5 µm
Endfitting Type	Parker-style
Pore Size	130 Å
QC Tested	Glycan
Format	Column
Surface Area	185
System	UHPLC
Particle Technology	BEH
USP Classification	L68
Inner Diameter	2.1 mm
Length	150 mm
Carbon Load	12 %
UNSPSC	41115709
Application	Glycan
Brand	XBridge
Product Type	Columns
Units per Package	1 pk

FAQs

Question: What is the main application of XBridge Glycan BEH Amide XP?

Answer:HILIC separation and profiling of labeled released glycans, including structural glycan variants.

Question: How does the single column differ from the method validation kit?

Answer:The individual product supplies one 2.1 × 150 mm column; the validation kit contains three columns from different batches.

Question: What packing material and pore size are used?

Answer:The column contains 2.5 µm BEH hybrid particles with 130 Å pores and glycan-selective amide chemistry.

Application Note

Waters XBridge Glycan BEH Amide XP Column provides 2.1 × 150 mm HILIC separation of released glycans using 2.5 µm hybrid BEH particles and 130 Å pores. Its glycan-specific amide surface supports separation of structurally related carbohydrate species that may differ in branching, galactosylation, fucosylation or sialylation. The stationary phase is quality-control tested with a labeled glycan performance standard to support consistent glycan profiles across batches. The 2.1 mm format is practical for fluorescence-based glycan mapping on low-dispersion LC systems and can also be incorporated into appropriately optimized LC-MS workflows. Mobile-phase gradients, labeling chemistry and column temperature govern retention and selectivity. One analytical column is supplied. This individual column shares its separation chemistry with the XBridge Glycan Method Validation Kit but does not supply the three independent manufacturing batches included in that kit.

Ordering

Notice

Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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