Waters, 186009524, ACQUITY Premier Glycan BEH Amide, 130Å, 1.7 µm, 2.1 x 150 mm Column, 1/pk

Name: ACQUITY Premier Glycan BEH Amide, 130Å, 1.7 µm, 2.1 x 150 mm Column, 1/pk
Brand: Waters
SKU: 186009524

Catalog Number: 186009524

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Category

HILIC Columns

Pack Size1/pk

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Description

The ACQUITY Premier Glycan BEH Amide 130Å, 1.7 µm, 2.1 x 150 mm Columns are designed for HILIC-mode separations of 2-aminobenzamide (2-AB), 2-aminobenzoic acid (2-AA), or Waters RapiFluor-MS (RFMS) (p/n: 176003635) labeled glycans. Each batch of Glycn BEH Amide 130Å, 1.7 µm material is specifically quality control tested with the Waters Glycan Performance Test Standard (p/n: 186006349) to help ensure batch to batch consistency. The column hardware utilizes MaxPeak High Performance Surface (HPS) that reduces unwanted analyte surface interactions and greatly improves peak shape, recovery, and reproducibility.

Suggested use: Glycan Analysis, Glycopeptide analysis

Waters is phasing out Certificate of Analysis (COAs) for eCords. All column documents are now available at www.waters.com/coa

Specifications

General

Chemistry	Amide
Separation Mode	Hydrophilic Interaction (HILIC)
Particle Substrate	Hybrid
pH Range Min	2 pH
pH Range Max	11 pH
Temperature Limits	90 C
Maximum Pressure	15000 psi (1034 Bar)
Endcapped	No
Silanol Activity	Low
Particle Shape	Spherical
Particle Size	1.7 µm
Endfitting Type	Parker-style
Pore Size	130 Å
QC Tested	Glycan
Format	Premier Column
Surface Area	185
System	UPLC, UHPLC
Particle Technology	BEH
Technique	LC/MS
USP Classification	L68
Inner Diameter	2.1 mm
Length	150 mm
Carbon Load	12 %
eCord	Yes
UNSPSC	41115709
Application	Glycan
Brand	ACQUITY UPLC
Product Type	Columns
Units per Package	1 pk

FAQs

Question: Which separation mode does the Premier Glycan BEH Amide column use?

Answer:It uses hydrophilic interaction liquid chromatography (HILIC) with an amide stationary phase.

Question: What are the particle and column dimensions?

Answer:The column is 2.1 × 150 mm with 1.7 µm particles and 130 Å pores.

Question: What does MaxPeak surface technology do?

Answer:It reduces unwanted interaction between glycans and metal components in the column flow path.

Application Note

Waters ACQUITY Premier Glycan BEH Amide is a hydrophilic-interaction (HILIC) UHPLC analytical column developed for released glycan characterization. The 2.1 × 150 mm column uses 1.7 µm, 130 Å bridged-ethyl hybrid particles with amide chemistry to retain fluorescently labeled glycans, including 2-AB, 2-AA and RapiFluor-MS derivatives. MaxPeak High Performance Surface hardware reduces unwanted interactions with metal surfaces, supporting sensitive LC and LC–MS workflows. Its glycan-tested packing differs from an ordinary reversed-phase C18 column. The column is supplied individually for use in compatible high-pressure LC methods.

Ordering

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