Waters, 186006938, ACQUITY UPLC Peptide CSH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 15K, 1/pk

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The Waters ACQUITY UPLC Peptide CSH C18 analytical column is optimized for reversed-phase peptide separations using charged-surface hybrid (CSH) particle technology. Its 1.7 µm particles have 130 Å pores in a 2.1 × 150 mm column format, with an application molecular-weight range of approximately 1–15 kDa. The CSH surface includes a controlled low density of positive charge that can improve peptide peak shape with formic acid or trifluoroacetic acid mobile phases. This makes the phase useful in LC and LC-MS workflows where ionic interactions and ion-pairing-agent selection affect chromatographic performance. The fine particle size and long column require a compatible UPLC/UHPLC system and careful attention to pressure, injection solvent and sample cleanliness. Equilibrate under the method's initial gradient conditions and monitor peak tailing, retention reproducibility and resolution using a representative peptide mixture. When contamination risk is high, pair the analytical column with an appropriately matched VanGuard pre-column rather than replacing the full analytical bed prematurely.