Note: The same column is also available with the Waters mAb Size Variant Standard at no additional cost. (p/n: 176005072) Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
Suggested Use: Gel Filtration, Size Exclusion Chromatography, Aggregate, Monomer and Fragment analysis, Antibody Conjugates
Waters is phasing out Certificate of Analysis (COAs) for eCords. All column documents are now available at www.waters.com/coa
| Chemistry | SEC |
|---|---|
| Separation Mode | SEC |
| Particle Substrate | Hybrid |
| pH Range Min | 2.5 pH |
| pH Range Max | 8 pH |
| Temperature Limits | 60 C |
| Maximum Pressure | 11000 PSI (759 bar) |
| Endcapped | No |
| Bonding Technology | BEH-PEO |
| Silanol Activity | Low |
| Molecular Weight Range Min | 10000 |
| Molecular Weight Range Max | 650,000 |
| Particle Size | 1.7 µm |
| Endfitting Type | Parker-style |
| Pore Size | 250 Å |
| QC Tested | Protein |
| Format | Online Column |
| Surface Area | 174 |
| System | UHPLC, UPLC, HPLC |
| Particle Technology | BEH |
| Technique | LC |
| USP Classification | L33, L59 |
| Inner Diameter | 4.6 mm |
| Length | 300 mm |
| Carbon Load | 12 % |
| eCord | Yes |
| UNSPSC | 41115709 |
| Application | Protein |
| Brand | ACQUITY |
| Product Type | Columns |
Answer:4.6 × 300 mm, 1.7 µm particles and 250 Å pores.
Answer:Separation of protein monomers, aggregates and fragments, including monoclonal antibodies.
Answer:About 10,000–650,000 Da.
The Waters ACQUITY™ Premier Protein SEC column separates protein size variants, including antibody monomers, aggregates and fragments, using 250 Å pore, 1.7 µm BEH-PEO hybrid particles. Its 4.6 × 300 mm hardware incorporates MaxPeak Premier high-performance surfaces to minimize unwanted secondary interactions between protein analytes and the column flow path. The approximately 10–650 kDa working molecular-weight range supports characterization of monoclonal antibodies and related biopharmaceuticals. This size-exclusion column is quality-control tested with a monoclonal antibody size-variant standard and is intended for low-dispersion LC and UPLC SEC methods. It is supplied as one column, not as a column-and-standard bundle.
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
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| Units per Package | 1 pk |
|---|