Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay type:Quantitative,
Sensitivity:> 0.2 nM,
Range:0.2 - 10 nM,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
Adipogenesis Detection Assay Kit (Colorimetric/Fluorometric) (ab102513) quantifies triglyceride accumulation in cells and tissues. In the assay, triglycerides are efficiently solubilized then hydrolyzed to glycerol which is subsequently oxidized to convert the probe to generate color (ODmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The kit can detect 0.2 - 10 nmol of triglyceride in <1,000 differentiated 3T3-L1 cells. The high detection sensitivity and the convenient microplate assay format make the kit a convenient tool for studying the effect of adipogenesis inducers or to screen inhibitor compounds.
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This product is manufactured by BioVision, an Abcam company and was previously called K610 Adipogenesis Colorimetric/Fluorometric Assay Kit. K610-100 is the same size as the 100 test size of ab102513.
Adipogenesis is the process of differentiation of different cell types into adipocytes, the primary fat storage cell type. The accumulation of adipocytes is the basis for obesity, a significant risk factor in many diseases, including diabetes, atherosclerosis, cancer and cardiovascular disease, etc. Adipocytes accumulate triglycerides, in the form of lipid droplets which can be measured.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Adipogenesis also known as fat cell differentiation refers to the process of creating mature adipocytes from precursor cells such as mesenchymal stem cells. The process involves different stages marked by the expression of several key transcription factors like C/EBPα and PPARγ. These factors regulate the conversion of preadipocytes into fully functional adipocytes. Primary sites for adipogenesis include white and brown adipose tissues and this process can be studied using various detection methods including fluorometric detection and adipored assays.
Biological function summary
The formation of adipocytes involves the activation of specific gene networks that govern lipid accumulation and insulin sensitivity. Adipogenesis plays a significant role in energy storage and homeostasis by having the function of sequestering lipids in the form of triglycerides. It involves complex signaling pathways where multiple proteins interact and coordinate to facilitate lipid droplet formation and adipocyte maturation.
Pathways
Adipocyte development is tightly integrated into the insulin signaling and lipid metabolism pathways. Within these pathways adipogenesis interacts with proteins like AKT and mTOR which are important for promoting lipid accumulation and maintaining energy balance. The pathways form a coordinated system that ensures efficient energy storage and mobilization depending on metabolic demands.
Improper regulation of adipogenesis is linked with metabolic diseases such as obesity and type 2 diabetes. Dysregulated adipocyte formation can contribute to ectopic lipid accumulation and insulin resistance. The process of adipogenesis influences pathological states through proteins such as TNF-α which is elevated in these disorders and negatively impacts adipocyte function. Proper understanding and manipulation of this process offer potential therapeutic avenues for controlling obesity-related complications.
