Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Tissue Extracts, Cell culture supernatant, Serum, Saliva,
Assay type:Enzyme activity,
Sensitivity:= 10 mU/well,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Aspartate Aminotransferase Activity Assay Kit (AST assay kit) ab105135 provides a rapid, simple, sensitive and reliable assay for AST activity. Aspartate aminotransferase (AST) is also known as Aspartate transaminase and Glutamate-oxaloacetate transaminase (GOT).
AST assay principle
In the AST assay, aspartate aminotransferase acts on aspartate and alpha-ketoglutarate, transferring an amino group from aspartate to α-ketoglutarate, and producing glutamate and oxaloacetate. Glutamate dehydrogenase then acts on the glutamate in an NAD-coupled reaction that produces a colorimetric readout (450 nm).
AST Activity assay protocol summary
- Add samples and standards to wells
- Add reaction mix
- Incubate at 37°C and analyze with microplate reader after 10 mins and then again after 60 mins
How other researchers are using ab105135
AST Activity Assay Kit ab105135 has been used in a variety of sample types including:
Mouse cirrhosis-hepatocellular carcinoma
Diabetic rat blood samples
Blood sample from obese rats
References: 1-Awad B et al. 2023, 2-Umar U et al. 2023, 3-Liao J et al. 2023
Related and recommended products
For fluorometric detection, we recommend Aspartate Aminotransferase Activity Assay Kit (Fluorometric)
ab138878
AST Assay Kit / Aspartate Aminotransferase Activity Assay Kit ab105135 is often used with ALT Assay Kit / Alanine Transaminase Activity Assay Kit
ab105134
as a marker for liver damage.
This product was previously called K753 Biovision Aspartate Aminotransferase (AST or SGOT) Activity Colorimetric Assay Kit. Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aspartate Aminotransferase (AST) also known as aspartate transaminase or aspartate transferase is an enzyme important for amino acid metabolism. Mechanically it catalyzes the reversible transfer of an amino group from aspartate to α-ketoglutarate forming oxaloacetate and glutamate. AST has a molecular mass of approximately 92 kDa and is expressed in various tissues with high levels found in liver heart muscle and kidneys. Its presence in these tissues highlights its importance in cellular metabolic processes.
Biological function summary
AST facilitates the interconversion between aspartate and oxaloacetate playing a role in the amino acid and urea cycles. Though not part of a large complex AST works closely with similar enzymes such as alanine aminotransferase (ALT) to maintain amino acid balance and support energy production. This enzymatic activity is important in nitrogen metabolism and glutamate use which are necessary for synthesizing other essential molecules within the cell.
Pathways
AST participates in the citric acid cycle and malate-aspartate shuttle enabling efficient energy production and NADH transport. The enzyme assists in converting oxaloacetate a pivotal intermediate in the citric acid cycle to keep the cycle active ensuring efficient cellular respiration. Additionally in the malate-aspartate shuttle AST works alongside malate dehydrogenase to facilitate the transfer of reducing equivalents across the mitochondrial membrane which is essential for ATP generation.
Elevated AST levels often indicate liver damage such as in hepatitis or cirrhosis reflecting its significant tissue expression. The enzyme is also linked to myocardial infarction as damaged heart muscle releases AST into the bloodstream. In these conditions AST serves as a biomarker for tissue damage often in conjunction with ALT levels allowing clinicians to assess the extent of liver or cardiac injury.
