Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Reacts with:Mouse, Human,
Sensitivity:= 2 ng/well,
Assay time:3h,
Assay Platform:Microplate reader
Product details:
Phosphorylation of histone H3 at serine 10 is conserved through eukaryotes, and an increase in phosphorylation has been shown to correlate with gene activation and cell growth. H
istone H3 (phospho S10)
is regulated by the cell cycle and has been used as a mitotic marker. In vitro studies have shown that H3 (phospho S10) is coupled to acetylation at the nearly K14 residue and that it is negatively impacted by H3 (methyl K9).
Histone H3 (phospho S10) Assay Kit (Colorimetric) (ab115127) allows the user to measure global histone H3 (phospho S10) quickly and consistently. The kit is ready-to-use and provides all the essential components needed to carry out a successful assay experiment and it suitable for specifically measuring global histone H3 phophorylation at Ser10 using a variety of mammalian cells including fresh and frozen tissues, cultured adherent and suspension cells.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
