Description
Key facts
Detection method:Colorimetric,
Sample types:Saliva, Urine, Plasma, Tissue, Suspension cells, Serum, Adherent cells,
Assay type:Quantitative,
Sensitivity:>= 0.2 ng/well,
Assay time:4h,
Assay Platform:Microplate reader
Product details:
Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
It can be paired to use Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies
, for simultaneous quantification of both methylated and hydroxymethylated DNA.
DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in
5-methylcytosine (5-mC)
. In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
