Description
Key facts
Detection method:Colorimetric,
Sample types:Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 13 ng/mL,
Range:31.25 - 2000 ng/mL,
Assay time:1h 30m,
Assay Platform:Microplate
Product details:
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is a rapid single-wash 90-min ELISA kit to measure Human alpha 1 Antitrypsin in serum and plasma. This SimpleStep ELISA® sensitivity is 0.02 ng/mL.
How the assay works
Human alpha 1 Antitrypsin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Human IgM, human IgA and human IgE were prepared at 10 ng/mL and 250 ng/mL in Sample Diluent NS and assayed for cross reactivity. No cross-reactivity was observed for IgM or IgE at either concentration with a mean OD deviation from background of -0.01. No cross-reactivity was observed for IgA at 10 ng/mL and only 0.4% cross-reactivity at 250 ng/mL of IgA.
Purified human IgG was assayed at 5 ng/mL in the presence and absence of 250 ng/mL of human IgM, human IgA and human IgE to determine interference. After background subtraction, recovery of human IgG was observed at a mean of 98% with a standard deviation of 0.06.
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers have used Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 has been used in publications to study bioengineered liver grafts for treating acute liver failure
, alpha 1 antitrypsin for predicting acute kidney injury after cardiopulmonary bypass
, and cystatin C correlation with alpha-1 antitrypsin in asthma
References:
Jeff J Ross et al. 2022 PMID: 37892931,
Jun Ai et al. 2019 PMID: 32030221,
Mohamad Sadikin et al. 2023 PMID: 38094096
Save your precious samples
Our 384-well Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) only requires a maxmum of 12.5µL
Related and recommended products
Human alpha 1 Antitrypsin ELISA Kit (SERPINA1) ab189579 is commonly used to study liver function and inflammation.
Other kits for measuring liver function and inflammation include:
- Human Albumin ELISA kit
ab179887
- Human ALT ELISA kit
ab234578
- Human AST ELISA Kit
ab263881
alpha 1 Antitrypsin (SERPINA1) is a serine protease inhibitor found in a variety of biological fluids. While it can inhibit trypsin, chymotrypsin, and plasminogen activator, it's main target is elastase. Alpha 1 antitrypsin plays a critical role in the lung by inhibiting the activity of elastase, produced by neutrophils during inflammatory responses. The aberrant form plays a role in coagulation and insulin induced NO synthesis. Alpha 1 antitrypsin deficiency results in emphysema and may also result in liver disease.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
