Description
Product details
What is this antibody validated in? Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Mouse samples. What is the molecular weight of Cleaved Caspase-3? Anti-Cleaved Caspase-3 [EPR21032] (ab214430) specifically detects a band for Cleaved Caspase-3 (UniProt: P70677) at a molecular weight of 31kDa. Trusted by the scientific community Anti-Cleaved Caspase-3 [EPR21032] (ab214430) was first used in a scientific publication in 2018 and has been cited over 110 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR21032] also available for your convenience: ab214430, Carrier free - ab231289
Patented technology Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Caspase 3 acts as an executioner enzyme in the process of programmed cell death. Its cleavage from the inactive zymogen form into the active form triggers apoptotic pathways ensuring the removal of damaged or unnecessary cells. Cleaved caspase operates within a proteolytic cascade often in conjunction with other caspases such as caspase 9. Once activated caspase 3 targets and cleaves specific cellular substrates contributing to the characteristic morphological changes and DNA fragmentation associated with apoptosis.
Pathways
Cleaved caspase 3 is significant within the intrinsic and extrinsic apoptotic pathways. It acts downstream of initiator caspases such as caspase 9 in the intrinsic pathway where its activation involves mitochondrial signals. In the extrinsic pathway it closely associates with caspase 8 mediating cell death signals from extracellular triggers. These pathways ensure that cleaved caspase 3 fulfills its role as a critical mediator of apoptosis linking upstream death signals to cellular dismantling during the apoptosis process.