Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2,
Disease:Adenocarcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GSTT2B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GSTT2 also known as Glutathione S-transferase Theta-2 belongs to the GST superfamily of enzymes. This protein has an approximate molecular mass of 27 kDa. GSTT2 is involved in the conjugation of reduced glutathione to various electrophilic compounds facilitating the detoxification process. It is expressed in several tissues with notable presence in the liver kidney and testis. GSTT2 plays a role in processing many xenobiotic substances enhancing cell resistance to oxidative stress.
Biological function summary
GSTT2 facilitates the detoxification process by catalyzing reactions that neutralize harmful compounds. It catalyzes the conjugation of glutathione to form less reactive electrophilic or hydrophobic substances. GSTT2 does not function as part of a larger complex within cells but has an important role in maintaining the redox balance. The enzyme contributes to cellular defense mechanisms ensuring proper cellular functions in stressful environments.
Pathways
The activity of GSTT2 has connections to the glutathione metabolism and drug metabolism pathways. These pathways are important for neutralizing endogenous and exogenous toxins. In the glutathione metabolism pathway GSTT2 works in conjunction with other GST enzymes like GSTP1 and GSTA1 to manage oxidative stress. The protein indirectly supports cellular detoxification processes influencing how effectively a cell can endure and adapt to toxic insults.
GSTT2's role in detoxification links it to conditions such as cancer and liver diseases. Altered GSTT2 expression has associations with the development of hepatocellular carcinoma. The protein interacts with related GST family members like GSTM1 and GSTP1 which play roles in modulating cancer susceptibility. Due to its role in processing carcinogens and protecting tissues from oxidative damage GSTT2 presents as a noteworthy protein in understanding disease mechanisms and potential therapeutic targets.
