Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and Insertion of the selection cassette in exon 3,
Disease:Adenocarcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GPSM2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LGN also known as GPSM2 (G-protein signaling modulator 2) is a protein involved in cellular processes related to signaling and cell polarity. It has a molecular mass of approximately 80 kDa. LGN is expressed in various tissues including the central nervous system where it plays a role in neural cell functions. This protein acts mechanically by interacting with G-proteins and aiding in proper signal transduction.
Biological function summary
The protein LGN influences cell division by regulating spindle orientation which is essential for accurate cell fate determination. LGN is a part of a complex with the proteins NuMA (nuclear mitotic apparatus protein) and dynein-dynactin which assists in the positioning of the mitotic spindle during cellular division. This activity ensures that cells divide correctly maintaining tissue organization and function.
Pathways
LGN is important in asymmetric cell division and cellular signaling. It is involved in the Wnt signaling pathway which is known for its role in cell growth and development. Additionally LGN interacts with G-proteins modulating their activity to facilitate proper signal transduction within these pathways. The influences on these pathways highlight the protein's necessity in maintaining cellular organization and signaling fidelity.
LGN is linked to certain neurodevelopmental disorders such as microcephaly where patients exhibit reduced brain size due to abnormal cell division. It also connects to certain types of cancer through its role in maintaining correct spindle orientation and polarity which is important for preventing uncontrolled cell proliferation. LGN's interaction with proteins like NuMA and G-proteins remains a focal point in understanding its impact on these conditions.
