Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 3,
Disease:Adenocarcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HENMT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HENMT1 also known as HEN1 methyltransferase homolog 1 is an enzyme with a molecular mass of approximately 55 kDa. It is expressed in the germ cells of both mammals and plants. This enzyme belongs to the small RNA methylation family and it plays an important role in the modification of small RNAs specifically piwi-interacting RNAs (piRNAs). HENMT1's primary function is transferring a methyl group to the 3' end of piRNAs protecting them against uridylation and exonucleolytic degradation keeping their integrity and stability necessary for their function.
Biological function summary
The enzyme HENMT1 participates in piRNA biogenesis. These piRNAs are part of a larger ribonucleoprotein complex that includes Piwi proteins which are essential for transposon silencing in the germline. This complex ensures the epigenetic and post-transcriptional silencing of transposable elements consequently protecting genomic integrity. HENMT1's activity is fundamental for safeguarding the genome from potential aberrations caused by active transposons.
Pathways
HENMT1 plays a significant part in the piRNA pathway which is important for gene silencing in the germline. Through this pathway it contributes to the protection of the genome against transposable elements by working alongside proteins like Piwi. The RNA interference (RNAi) pathway also involves HENMT1 although indirectly as RNAi and piRNA pathways share some mechanistic similarities in their mode of silencing unwanted genetic elements.
HENMT1 is connected to male infertility and certain cancers. Defects or malfunctions in HENMT1 can disrupt piRNA production leading to genomic instability and contributing to infertility in males. Additionally irregularities in its function might link it to cancer development possibly through faulty regulation of transposons. Other proteins like Piwi are relevant to understanding HENMT1's involvement in these conditions due to their collaborative function in the piRNA complex.
