Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp deletion in exon 3 and 4 bp deletion in exon 3
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GNG4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The G-protein gamma subunit 4 also known as GNG4 is a component of the heterotrimeric G-protein complex. It has a molecular mass of approximately 7.9 kDa. This protein is typically expressed in various tissues including the brain and spinal cord. Functionally GNG4 is involved in signal transduction processes by forming a complex with the G-protein beta subunit playing a critical role in transmitting signals from G-protein-coupled receptors (GPCRs) to downstream effectors.
Biological function summary
GNG4 participates in the modulation of intracellular signal pathways. It forms an integral part of the G-protein complex which includes another subunit known as GNB1. This complex is necessary for proper functioning of GPCR-mediated signaling. The G-protein complex influences various cellular responses by affecting ion channels enzymes and other intracellular pathways. The presence of GNG4 in neuronal tissues suggests its role in neurotransmission and the potential influence on neurologic function.
Pathways
GNG4 plays a role in the adenylyl cyclase signaling pathway and the phospholipase C pathway. In both pathways GNG4 together with GNB1 regulates the conversion of extracellular signals into intracellular actions. These actions include cyclic AMP production or calcium ion release which subsequently affect cellular activities. This involvement links GNG4 to various cellular processes by mediating inputs from diverse signals through its interaction with GPCR-related pathways.
GNG4 is associated with conditions such as neuropsychiatric disorders and cancer. The aberrant expression or mutation of GNG4 can disrupt normal signaling pathways contributing to disease pathology. In neuropsychiatric disorders GNG4 interacts with dopamine receptor pathways affecting dopaminergic signaling; this connection suggests a possible link to conditions like schizophrenia. In cancer altered GNG4 expression has been observed in some tumor types indicating its potential involvement in tumorigenesis through signaling pathways linked to other proteins such as Ras.
