Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-EIF2D, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The eukaryotic translation initiation factor 2D (EIF2D) also known as DAP1 or MCTS1 is a human protein involved in initiating translation of mRNA into proteins. It has a molecular weight of approximately 54 kDa. EIF2D is expressed in various tissues showing its involvement in diverse cellular processes. Mechanically EIF2D facilitates the recycling of ribosomal subunits ensuring efficient protein synthesis. It performs its role by helping the ribosome subunits which have dissociated after termination to be reused for new rounds of translation.
Biological function summary
EIF2D influences protein synthesis through its role in translation initiation and ribosome recycling. It may operate as a part of a complex potentially interacting with other factors involved in translation setup. This role is fundamental to maintaining cellular homeostasis and responding to cellular stress by adjusting protein production rates. EIF2D's interaction with the ribosome and its factors points to its active involvement in the complex regulation of cellular protein levels.
Pathways
EIF2D takes part in the translation initiation pathway which involves other proteins such as eIF1 and eIF2. These factors work together to ensure proper recognition of start codons on mRNA strands. EIF2D also fits into the mRNA surveillance pathway which is responsible for ensuring the fidelity of mRNA translation. The accuracy of this process is critical for proper cellular function and response to stress or damage signals.
EIF2D has associations with cancer and metabolic disorders. Its role in protein synthesis regulation impacts how cells grow and proliferate pathways often found altered in cancerous cells. EIF2D may be linked to components like mTOR which is involved in cell growth and metabolism. Additionally EIF2D's function in responding to cellular stress links it to metabolic syndromes where the shifts in protein synthesis can lead to pathological effects.
