Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-DGUOK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target 'dGK' also known as deoxyguanosine kinase is an enzyme that plays an important role in the salvage pathway of nucleotide synthesis. It is a mitochondrial kinase with a known mass of approximately 29 kDa. The enzyme catalyzes the phosphorylation of purine deoxyribonucleosides such as deoxyguanosine to their monophosphate forms. dGK is expressed mainly in mitochondria-rich tissues like the liver muscle and brain indicating its importance in nucleoside metabolism in these areas.
Biological function summary
Deoxyguanosine kinase contributes significantly to the maintenance of mitochondrial DNA by ensuring the supply of deoxyribonucleotide triphosphates (dNTPs). It does not function as part of a larger protein complex working independently to convert purine deoxynucleosides in a rate-limiting step. The enzyme ensures that cellular processes requiring DNA replication and repair can proceed highlighting its importance in cellular functionality and homeostasis.
Pathways
Deoxyguanosine kinase participates in the nucleotide salvage pathway which is pivotal for recycling nucleosides into nucleotide pools. This pathway complements the de novo synthesis pathway providing a rapid response mechanism for nucleotide demand. dGK operates alongside other kinases like thymidine kinase which similarly act to phosphorylate nucleosides. This interconnection facilitates the balance of nucleotide pools within the cell important for effective DNA synthesis and repair.
Mutations or deficiencies in deoxyguanosine kinase can lead to mitochondrial DNA depletion syndromes. Such conditions result in a variety of clinical manifestations including muscle weakness and neurological issues. dGK is linked to disorders like mitochondrial neurogastrointestinal encephalopathy where faulty dNTP pools cause progressive multisystemic symptoms. The disturbance in deoxyguanosine kinase's function emphasizes its role in maintaining cellular energy homeostasis and genome integrity.
