Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 20 bp deletion in exon 2
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ELOF1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ELOF1 known as Elongation Factor Protein 1 plays a role in the transcription process. It assists RNA polymerase II with transcription elongation and maintaining transcript integrity. ELOF1 has a molecular weight of approximately 15 kDa. It is expressed in various tissues with higher levels found in active proliferating cells due to its role in gene expression regulation.
Biological function summary
ELOF1 supports the elongation phase of transcription and interacts with components of the transcription machinery. It forms part of a complex with other elongation factors helping RNA polymerase II proceed along DNA without stalling. ELOF1 ensures efficient synthesis of messenger RNA affecting the transcriptional output of numerous genes across different cellular processes.
Pathways
The influence of ELOF1 extends to key transcriptional and signaling pathways like the mRNA processing pathway. ELOF1 also connects closely to other transcription elongation factors like SPT6 affecting how cells respond to transcriptional cues. These connections are significant in maintaining proper gene regulation and expression in reaction to various intracellular signals.
ELOF1's interplay with transcription regulation links it to cancer and neurodegenerative diseases. Changes in ELOF1 expression or function can disrupt normal transcriptional elongation contributing to the uncontrolled growth of cancer cells. Additionally dysfunction in ELOF1 influences neurodegenerative processes likely through its regulatory effect on gene expression. It is associated with proteins like RNA polymerase II which play roles in these disease pathways.
