Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 8 bp deletion in exon 1
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-AK2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The adenylate kinase 2 also known as AK2 is an enzyme that catalyzes the conversion of adenine nucleotides within the cell. It facilitates the reversible conversion of ADP into ATP and AMP playing a critical role in cellular energy homeostasis. AK2 weighing approximately 26 kDa is primarily found in the mitochondrial intermembrane space of various tissues including liver muscle and brain. Its expression allows for efficient energy transfer essential for normal mitochondrial function and cellular metabolism.
Biological function summary
AK2 functions in energy transfer and regulation in the cell serving as an important component in maintaining cellular ATP levels. AK2 is an integral part of the mitochondrial adenylate kinase system which manages energy flux in cells. By balancing the concentrations of ATP ADP and AMP AK2 directly influences cellular energy state and metabolic activity. The enzyme also supports nucleotide homeostasis therefore facilitating processes such as signal transduction and nucleic acid synthesis.
Pathways
AK2 is involved in the adenylate kinase pathway important for energy metabolism. It coordinates with other enzymes like AK1 and AK3 ensuring efficient energy distribution in the cell. AK2 also interfaces with the apoptosis signaling pathways where it plays a part in regulating programmed cell death. The interaction of AK2 within these pathways emphasizes its essential role in maintaining cellular energy balance and undergoing apoptosis when necessary.
AK2 mutations link to rare conditions like reticular dysgenesis a severe form of combined immunodeficiency. The disorder arises when AK2 fails to initiate proper energy transfer necessary for white blood cell development. AK2 is also related to mitochondrial disorders where energy production gets critically impaired manifesting in muscle weakness and neurodegenerative symptoms. The enzyme's connection to proteins involved in energy pathways suggests its potential contribution to the progression of such diseases.
