Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ERP29, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ERp29 also known as Endoplasmic Reticulum Resident Protein 29 is a protein with a molecular weight of about 29 kDa. It acts mechanically by aiding protein folding and assembly in the endoplasmic reticulum (ER). This protein is expressed in many tissues but more in the brain liver and pancreas. ERp29 contributes to cellular homeostasis by facilitating the proper folding of newly synthesized proteins within the ER.
Biological function summary
This protein assists in maintaining ER function by preventing the accumulation of misfolded proteins. ERp29 is part of the protein disulfide isomerase family which helps in forming disulfide bonds in proteins ensuring their correct structure and function. It does not form part of a larger complex but interacts with other proteins to manage stress and maintain cellular function. These interactions help in the secretory pathway important for processing proteins.
Pathways
ERp29 plays an innovative role in the Unfolded Protein Response (UPR) a cellular reaction to ER stress. It works alongside proteins like GRP78 within the UPR to mitigate stress's harmful effects by enhancing the protein folding capacity of the ER. Additionally ERp29's involvement in the protein disulfide isomerase family connects it to the oxidative protein folding pathway which is essential for maintaining redox balance in the ER.
ERp29's misregulation connects to neurodegenerative disorders such as Alzheimer's disease. It relates through its protein folding and stress response roles impacting cellular survival and function. Proteins like amyloid precursor protein (APP) show interaction with ERp29 in this context. Moreover in cancer biology ERp29 expression levels correlate with the progression of certain types of tumors as it influences pathways related to cell survival and apoptosis.
