Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 14 and 4 bp insertion in exon 14,
Disease:Carcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-DDX60L, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DDX60L also known as DExD/H-box helicase 60-like is a protein involved in the unwinding of RNA molecules. With a mass of approximately 147 kDa it plays a significant role in RNA metabolism. This protein is mostly expressed in immune-related tissues such as the spleen and lymph nodes. DDX60L through its helicase activity binds and processes RNA which impacts the host antiviral response mechanism.
Biological function summary
The functions of DDX60L include sensing viral RNA and activating downstream immune responses. It associates with other similar proteins to form a complex that initiates sensing pathways for RNA viruses. This engagement leads to the activation of innate immune responses which are essential for detecting and responding to infections. DDX60L is part of signaling complexes contributing to the regulation and modulation of immune pathways.
Pathways
DDX60L plays a pivotal role in the RIG-I-like receptor (RLR) signaling pathway. This pathway detects viral RNAs and initiates antiviral responses through interferon production. DDX60L interacts with proteins like RIG-I (retinoic acid-inducible gene I) to regulate the type I interferon signaling pathway enhancing the body's ability to fight viral infections. The protein contributes to maintaining cellular homeostasis under immune stress conditions.
DDX60L is linked to viral infections such as influenza and hepatitis. Its role in the antiviral response makes it a potential target for interventions designed to enhance immune responses against these infections. Additionally the protein's interaction with RIG-I suggests a contributory role in disorders related to dysfunctional immune responses potentially impacting autoimmune conditions where viral components play a part. Understanding DDX60L's function could inform therapeutic approaches to manage these viral-induced disorders.
