Description
Key facts
Form:LiquidSee storage information,
Storage buffer:pH: 6 - 8Constituents: Agarose beads, 20% Ethanol
Product details:
Product overview
MBP tagging of recombinant proteins can result in increased expression levels and higher solubility of the target protein, while allowing high purity recovery. Proper folding of the attached protein has also been shown to be promoted by the MBP tag. Due to the increased solubility, the tag can be extremely useful for recombinant proteins accumulated in an insoluble form (inclusion bodies).
Maltose Binding Protein Affinity Resin - Amintra (ab270538) is a chromatography medium for purifying proteins fused to maltose binding protein (MBP-tagged protein). The base matrix of this resin is a robust, highly cross-linked 6% agarose, optimized to give high physical and chemical stability and high binding specificity. The evenly sized beads ensure that MBP-tagged proteins elute in narrow peaks, minimising the need for further time-consuming concentration steps.
Affinity purification using Maltose Binding Protein Affinity Resin - Amintra takes place under physiological conditions, and mild elution is performed using maltose which preserve target protein activity. These mild conditions may even allow purification of intact protein complexes.
The characteristics of the resin:
Matrix
Highly crosslinked 6% agarose
Ligand
Dextrin
Capacity
>10 mg MBP tagged protein (80 kDa)/mL medium
Particle Size
45-165 μm
Maximum Pressure
0.3 MPa, 3 bar
pH Stability
Storage Buffer
20% ethanol
General notes
This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Maltose Binding Protein (MBP) Affinity Resin. Expedeon product code AMB0015 (15 mL Medium) and AMB00100 (100 mL of Medium) are the same as the current sizes: 15 mL and 100 mL, respectively.
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage duration-Up to 12 months, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C
