Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift: 98%,
Disease:Carcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CYP24A1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CYP24A1 also known as cytochrome P450 family 24 subfamily A member 1 is an enzyme with a molecular mass of approximately 56 kDa. It plays an important role in the catabolism of active vitamin D metabolites particularly in the hydroxylation of calcitriol. This enzyme is located mainly in the kidney but also in other tissues such as the placenta and the pituitary gland. Its expression is regulated by various physiological factors including calcium and phosphate levels.
Biological function summary
This enzyme modulates the levels of active vitamin D influencing calcium and phosphate homeostasis. By converting calcitriol into its inactive forms CYP24A1 prevents excessive vitamin D activity which affects bone mineralization and cellular processes. It does not function as part of a larger complex but interacts closely with other vitamin D-metabolizing enzymes.
Pathways
CYP24A1 is an integral component of the vitamin D metabolism pathway. It acts in concert with another enzyme CYP27B1 which activates vitamin D by hydroxylating calcidiol to calcitriol. Together they balance the hormone levels vital for various cellular functions. The enzyme also participates in calcium signaling pathways affecting cellular growth and differentiation.
Dysregulation of CYP24A1 has been associated with conditions such as hypercalcemia and certain cancers like colorectal cancer. Overexpression or mutations in this enzyme lead to altered vitamin D metabolism exacerbating disease states. In cancer CYP24A1 often interplays with proteins like VDR the vitamin D receptor influencing cancer cell proliferation and response to therapy.
