Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 100%,
Disease:Adenocarcinoma
Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ATG3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions--196°C, -80°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATG3 also known as Autophagy-related protein 3 is an essential enzyme in the autophagy process. Its molecular weight is approximately 36 kDa. This protein facilitates the conjugation of LC3 (microtubule-associated protein 1A/1B-light chain 3) by acting as an E2-like enzyme. It is mainly expressed in tissues with high rates of autophagy including liver heart and brain.
Biological function summary
ATG3 plays an important role in the elongation of the autophagic membrane. It operates as a part of the autophagy machinery working closely with ATG7 an E1-like enzyme to transfer phosphatidylethanolamine (PE) to LC3 converting it into LC3-II. This lipidated form of LC3 is essential in the expansion and closure of the isolation membrane which forms the autophagosome the core structure for degradation and recycling of cellular components.
Pathways
ATG3 is a central player in the macroautophagy pathway a process critical for cellular homeostasis. It interacts with ATG7 to facilitate the lipidation of ATG8 family proteins which are important for autophagosome formation. Moreover ATG3 also links to apoptosis pathways where its balance with pro-apoptotic proteins helps regulate cell death processes.
ATG3 has been implicated in neurodegenerative diseases like Alzheimer's. This relates partly to its role in protein clearance which is essential for preventing toxic aggregations. Altered ATG3 function can lead to accumulation of misfolded proteins exacerbating disease. Moreover ATG3's interaction with proteins in the cancer-related pathways such as p53 shows its involvement in tumorigenesis through its influence on cell survival and death mechanisms.
