Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,
Disease:Carcinoma
Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ERRFI1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MIG-6 also known as Gene 33 or ERRFI1 is a protein that functions as an adaptor/scaffold. It has an approximate mass of 70 kilodaltons and is found predominantly in epithelial tissues. MIG-6 acts as a negative regulator of the epidermal growth factor receptor (EGFR) signaling pathway. This regulation role involves binding to the tyrosine kinase domain of the EGFR which leads to a decrease in its catalytic activity. Besides its presence in epithelial tissues MIG-6 is also expressed in a variety of other tissue types including the liver and pancreas.
Biological function summary
MIG-6 plays a significant role in the regulation of cell proliferation differentiation and apoptosis. It is a part of a larger signal transduction network which is essential for controlling cellular responses to external stimuli. By acting as a brake on EGFR signaling MIG-6 helps to balance cell activities to prevent excessive growth and proliferation. The protein's impact extends to influencing the behavior of other signaling molecules within the cell contributing to meditative roles in maintaining cellular harmony.
Pathways
MIG-6 is integral to the EGFR signaling pathway and is closely associated with the MAPK (mitogen-activated protein kinase) pathway. Its regulatory function affects downstream signaling events that involve proteins like RAF MEK and ERK which are all critical in cell growth and survival. By interacting with these pathways MIG-6 modulates signal transduction cascades which allows for controlled cellular functions and responses. All these connections make it an important node for intracellular communication and pathway intersections.
Deficiencies or dysregulation of MIG-6 have been linked to cancer particularly in tumors that have heightened EGFR activity. The protein's ability to suppress EGFR signaling is essential in preventing unchecked cell proliferation that can lead to tumor growth. Additionally research has connected altered levels of MIG-6 with inflammatory disorders. Abnormal interactions between MIG-6 and EGFR can exacerbate inflammatory responses contributing to various chronic inflammatory conditions. Understanding these associations helps in the development of targeted therapies for conditions with altered EGFR signaling.
