Description
Key facts
Detection method:Fluorescent,
Assay type:Semi-quantitative,
Assay Platform:Microplate reader
Product details:
Multiplex IHC Detection kit (TSA amplification) is used for multiplex immunohistochemistry (mIHC) with tyramide signal amplification (TSA) on frozen tissue sections, which results in 10-50 times signal amplification compared with directly conjugated antibodies. The kit attaches horseradish peroxidase (HRP) to biotinylated antibodies in solution with click reaction using a processed form of streptavidin conjugated with HRP. The HRP–labeled biotinylated antibodies can then be used for multiplex TSA immunostainings.
TSA reagents are not provided in the kit.
The kit contains a HRP block buffer optimized for preserving the morphology of frozen tissue sections. The HRP block buffer rapidly quenches the HRP enzymatic activity after each cycle. The fast staining protocol allows 7-color IHC (DAPI + 6 biotinylated antibodies) using TSA to be completed in one day. For 7-color IHC appropriate choice of tyramide fluorochromes as well as microscopy systems optimized for multicolor analyses are needed.
Use this kit to:
Multiplex IHC tyramide signal amplification (TSA) on frozen tissue sections, this kit can also be used on paraffin embedded sections.
Technical notes
The large kit contains reagents to perform 200 immunostainings (100 μl staining volume). The small kit contains reagents to perform 70 immunostainings. There is no heat treatment between staining cycles, which allows multiplex TSA to be performed on frozen sections with preserved morphology. The kit shall be used with biotinylated antibodies The kit can be combined with other immunostaining protocols, such as primary or secondary antibodies conjugated with fluorochromes.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Please refer to protocols
